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Gametophyte development and conservation of Ctenitis submarginalis (Dryopteridaceae) in Buenos Aires Province, Argentina Boletín de la Sociedad
Ramos Giacosa,Juan P; Gorrer,Daniel A; Giudice,Gabriela E; Luna,María L.
The spore germination and gametophyte development of Ctenitis submarginalis from Punta Lara Natural Reserve, Buenos Aires province were studied as a contribution to the conservation of the species in this protected area. Spores collected from natural populations were sowing in Dyer medium and germinated in six day with a percentaje of 88 % fifteen days after sowing. The germination is Vittaria type and the gametophyte development is Aspidium type. The gametophyte of Ctenitis submarginalis is cordate, with a deep notch and well developed and symmetric wings. The gametangia appear 50 days after sowing. The sporophyte emerges 190 days after sowing and two different hairs were observed in the first lamina. The in-vitro spore germination is a successful method...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conservation; Ctenitis; Dryopteridaceae; Fern; Gametophyte.
Ano: 2017 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722017000400007
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In vitro spore culture and reproductive aspects of the annual fern Anogramma chaerophylla (Pteridaceae) Boletín de la Sociedad
Luna,María L; Yañez,Agustina; Ramos Giacosa,Juan Pablo; Gorrer,Daniel; Berrueta,Pedro C; Giudice,Gabriela E.
Anogramma chaerophylla belongs to a fern genus with annual sporophytes and potentially perennating gametophytes. In the studied area, Natural Reserve Punta Lara, the plants grow stressed mainly by pollution and the invasion of exotic species. As a part of a project on conservation of ferns inhabiting Buenos Aires province, the objectives of this work were to evaluate in Anogramma chaerophylla the conditions for in-vitro spore germination and to analyse the different stages of its reproductive cycle. Spores were sterilized in an aqueous solution 10 % of NaCIO during different times and then sown in Petri dishes with Murashige & Skoog medium, without the addition of sucrose. The dishes were kept under laboratory conditions, at 12 h light/ darkness...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anogramma chaerophylla; Micropropagation; Gametophyte; Tubercle; Ex-situ conservation.
Ano: 2016 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722016000400010
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Morfología de las esporas, desarrollo gametofítico y conservación de Thelypteris abbiattii y T. hispidula (Thelypteridaceae) de la reserva natural Punta Lara, Buenos Aires Boletín de la Sociedad
Ramos Giacosa,Juan Pablo; Giudice,Gabriela E; Pipo,Laura; Luna,María Luján.
Se estudiaron la morfología de las esporas y el desarrollo de los gametofitos de Thelypteris abbiattii y Thelypteris hispidula de material proveniente de la Reserva Natural Punta Lara (Buenos Aires, Argentina). Las esporas son monoletes, plegadas, con pliegues de margen equinado y con pliegue supralesural. La germinación de las esporas se realizó en medio de Dyer y se produjo entre los 7 a 12 días. En ambas especies la germinación es del tipo Vittaria y el desarrollo del gametofito tipo Aspidium. Los gametofitos adultos se observaron a partir de los 50 días, poseen forma cordiforme típica y son bisexuales. Dependiendo de las especies, la formación del esporofito se produce entre los 90 a 130 días. Se encontraron diferencias entre las especies analizadas en...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Thelypteris; Conservación; Esporas; Gametofito.
Ano: 2014 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722014000200008
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Morphology and ultrastructure of Schizaea fistulosa (Schizaeaceae) spores from Chile Boletín de la Sociedad
Ramos Giacosa,Juan P; Morbelli,Marta A; Giudice,Gabriela E.
The spores of Schizaea fistulosa from Chile were studied using light microscopy (LM), scanning (SEM) and transmission electron microscopy (TEM). The spores are monolete and elliptic in polar view. The major equatorial diameter is 71-85 µm and the polar diameter is 54-61 µm. The laesurae are 50-60 µm long and, in some cases, bifurcated. The sporoderm ultrastructure is first mentioned and described here. The exospore is two-layered in section, verrucate-tuberculate with single or fused elements forming short ridges. The perispore is single-layered, 10-30 nm thick and it is only visible under transmission electron microscopy. On the spore surface, numerous, single or fused, spheroids and nanospheroids of different sizes were observed attached to the perispore...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Schizaeaceae; Schizaea fistulosa; Spores; Morphology; Ultrastructure.
Ano: 2015 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722015000100003
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